Sampling methods for ventilator-associated pneumonia: Validation using different histologic and microbiological references

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Abstract
To validate sampling techniques (tracheobronchial aspirates, protected specimen brush, and bronchoalveolar lavage, both conventional and protected) for the detection of ventilator-associated pneumonia (VAP) and causative microorganisms according to different histologic and microbiological references. Immediate, multiple bilateral lung biopsy, postmortem study. Respiratory intensive care unit of a 1,000-bed teaching hospital. Twenty-five mechanically ventilated patients (>72 hrs) who died in our intensive care unit. Lung tissue histologic examination and quantitative cultures (16 specimens/patient). The following four references for the diagnostic techniques were used: histology of guided lung biopsy, histology of blind lung biopsy, combined guided and blind lung biopsy histology and microbiology of lung tissue, and microbiology of lung tissue. Sensitivities when histologic reference tests were used ranged from 16% to almost 40%, whereas specificity rates were always Conclusions The diagnostic performances of different diagnostic techniques strongly depend on the reference used. All techniques for detecting VAP are of limited value. Finding a balance between clinical judgment and microbiological results is crucial to appropriately manage patients with VAP.