Production of Dense Red Band Around Growth of Staphylococcus aureus on Blood Agar Plates

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Abstract
When certain strains of Staphylococcus aureus are grown on 10% whole human blood agar, a dense red band develops around the hemolytic zone of strains that are hemolytic for human erythrocytes and directly around the bacterial growth of strains that are not hemolytic. A substance is produced that diffuses through agar causing the development of the red bands. Sterile sections of agar taken from near the growth of S. aureus on plain agar and transplanted onto blood agar produce red bands showing that blood is not required for production of the diffusible factor but acts as indicator for demonstrating the red band phenomenon.

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