Mass spectrometric analysis of chitin oligosaccharides produced by Rhizobium NodC protein in Escherichia coli
Open Access
- 1 November 1995
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 177 (21) , 6282-6285
- https://doi.org/10.1128/jb.177.21.6282-6285.1995
Abstract
A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed. Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum bv. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.Keywords
This publication has 17 references indexed in Scilit:
- Structural identification of metabolites produced by the NodB and NodC proteins of Rhizobium leguminosarumMolecular Microbiology, 1994
- From peptidoglycan to glycoproteins: Common features of lipid-linked oligosaccharide biosynthesisFEMS Microbiology Letters, 1994
- Symbiotic host specificity between leguminous plants and rhizobia is determined by substituted and acylated glucosamine oligosaccharide signalsGlycobiology, 1994
- The Biosynthesis of Rhizobial Lipo-Oligosaccharide Nodulation Single MoleculesMolecular Plant-Microbe Interactions®, 1994
- Lipo-oligosaccharide nodulation factors: A new class of signaling molecules mediating recognition and morphogenesisCell, 1993
- Chitin and nodulationNature, 1991
- Expression Using the T7 RNA Polymerase/Promoter SystemCurrent Protocols in Molecular Biology, 1990
- Accumulation and decay of DG42 gene products follow a gradient pattern during Xenopus embryogenesisDevelopmental Biology, 1988
- BIOSYNTHESIS AND COMPOSITION OF GRAM-NEGATIVE BACTERIAL EXTRACELLULAR AND WALL POLYSACCHARIDESAnnual Review of Microbiology, 1985
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959