Application of Various Acid Dyes for Estimation of Protein in Milk by Dye-Binding
Open Access
- 1 May 1966
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 49 (5) , 455-459
- https://doi.org/10.3168/jds.s0022-0302(66)87896-7
Abstract
Acid dyes (23) were selected and compared for solubility in citrate buffer, stability of the solution, absorbance index and binding capacity with protein in milk. Acid-Orange-II, Resorcine-Brown, Xylene-Acid-Milling-Orange-2G and Diacid-Light-Green-GS which were excellent in binding capacity were finally chosen and field tests carried out for 6 months with individual Holstein and Jersey milks. Diacid-Light-Green-GS was much superior and Resorcine-Brown much inferior for estimating protein. The 3 days, except Resorcine-Brown, can be applied safely for mass protein testing of milk. Amido-Black-10B and Orange-G were relatively inferior both in protein binding and in desired properties of a dye.This publication has 1 reference indexed in Scilit:
- Dye-binding methods for estimation of protein in milkJournal of Dairy Research, 1961