Glutamine synthetase subunit mixing and regulation in Bacillus subtilis partial diploids.

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Abstract
A specialized transducing phage, phage SP.beta. c2 dglnA2, of B. subtilis was used to construct partial diploids with various glutamine auxotrophs. The overproduction of Mn-stimulated glutamine synthetase no longer occurred in the diploids. The kinetics of heat inactivation of the enzyme extracted from 2 diploids suggests that there was subunit mixing.