Quantitative evaluation ofEscherichia colihost strains for tolerance to cytosine methylation in plasmid and phage recombinants
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (9) , 3469-3478
- https://doi.org/10.1093/nar/17.9.3469
Abstract
Many strains of E. coli K12 restrict DNA containing cytosine methylation such as that present in plant and animal genomes. Such restriction can severely inhibit the efficiency of cloning genomic DNAs. We have quantitatively evaluated a total of 39 E. coli strains for their tolerance to cytosine methylation in phage and plasmid cloning systems. Quantitative estimations of relative tolerance to methylation for these strains are presented, together with the evaluation of the most promising strains in practical recombinant cloning situations. Host strains are recommended for different recombinant cloning requirements. These data also provide a rational basis for future construction of ''ideal'' hosts combining optimal methylation tolerance with additional advantageous mutations.This publication has 19 references indexed in Scilit:
- Efficient simplified cosmid cloning: Construction and characterization of cosmid vectors that carry the two cohesive end sites of λ phages arrayed in tandemAnalytical Biochemistry, 1989
- Molecular cloning and identification of protein products of rglB locus of Escherichia coliGene, 1988
- Genetic dissection of the methylcytosine-specific restriction system mcrB of Escherichia coli K-12Gene, 1988
- A novel mcrB-based Escherichia coli K-12 vector system and its use in analyzing the genetic determinants for the McrB nucleaseGene, 1988
- Escherichia coli sbcC mutants permit stable propagation of DNA replicons containing a long palindromeGene, 1988
- LINE-1: A mammalian transposable elementBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1987
- [12] Restriction and modification in vivo by Escherichia coli K12Published by Elsevier ,1987
- KpnI families of long, interspersed repetitive DNAs associated with the human β-globin gene clusterGene, 1982
- Analysis of gene control signals by DNA fusion and cloning in Escherichia coliJournal of Molecular Biology, 1980
- Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNAJournal of Molecular Biology, 1966