Investigations on the root nodule bacteria of leguminous plants

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Abstract
The decomposition of milk proteins, pure caseinogen, gelatin and the cell proteins of the nodule bacteria was studied both with living cultures of different strains of Rhizobium and with heavy suspensions in the presence of toluene. The breakdown of the different protein materials was found to be very slow and no differences could be noted between strains of different efficiency. There was a slow increase in the values for soluble and amino-N. Living rhizobia formed small amts. of ammonia from milk proteins and from gelatin; no formation of ammonia took place in the presence of toluene. No formation of ammonia could be demonstrated when the cell proteins of the rhizobia were used as substrates for living nodule organisms.

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