Analyses of Polyglycehol Esters of Fatty Acids Using High Performance Liquid Chromatography

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Abstract
Polyglycerol esters are important food emulsifiers available commercially as a mixture of various isomers. The need for analyses of these emulsifiers may arise at any stage of their production and use, and is important from the viewpoint of the health authorities. Polyglycerol esters of fatty acids can be analyzed by partition HPLC [high performance liquid chromatography] on a 25 cm column packed with 10 .mu.m LiChrosorb Diol. The isomers were eluted gradientally with n-hexane-isopropanol mixture within 60 min and the components monitored by UV-absorption at 220 nm. Monoglycerol and polyglycerol monoesters and polyglycerol polyesters of oleic acid were separated and evaluated.