8-Azido-ATP labelling of the FecE protein of the Escherichia coli iron citrate transport system
- 15 August 1992
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 95 (2-3) , 231-234
- https://doi.org/10.1016/0378-1097(92)90434-p
Abstract
The iron(III) transport system via citrate displays the characteristics of a binding protein-dependent transport mechanism through the cytoplasmic membrane. One of the five transport proteins, FecE, contains the two motifs found in ATP-binding proteins. Cloned overexpressed FecE was photoaffinity-labelled with [32P]8-azido-ATP. Labelling was inhibited by 1 mM ATP, ADP, GTP and less CTP, demonstrating the specificity of the reaction. It is suggested that ATP is the principal energy source for iron (III) citrate transport through the cytoplasmic membrane of Escherichia coli.Keywords
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