In vitro effects of ecdysterone on the spermatogonial cell cycle inLocusta

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Abstract
The effect of ecdysterone on specific phases of the cell cycle of Locusta migratoria migratorioides spermatogonia was assayed in vitro. An increase in labeling index was noted, indicating a decrease in duration of the G1 phase. On addition of the hormone to adapted organs in vitro, spermatogonial mitotic index increases rapidly, then declines to a basal level within a time period which approximates that of the G2 phase. Such a response indicates a removal of a G2/M inhibition by the hormone.