LIGHT SCATTERING FROM MOTILE BACTERIA

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Abstract
The «Intermediate scattering function» I(K, t) of light scattered by motile bacteria is shown to be I(K, t) = [MATH]Ps(V) dV, (1) where Ps(V) is the distribution of swimming speeds of the bacteria. Scattered spectra from motile E, Coli K12 bacteria have been investigated by laser light intensity correlation spectroscopy. [I(K, t)]2 is obtained from the data and its angular dependence is shown to agree with that predicted theoretically by eq. (1). In contrast, bacteria whose motility has been arrested by addition of 10-2 M CuCl2 are found to scatter light with a spectrum characteristic of large Brownian particles. The swimming speed distribution Ps(V) may be determined by performing appropriate Fourier inversions of the data taken from the motile bacteria. The manner by which the distribution changes when environmental factors are varied is indicated

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