DNA degradation and reduced recombination following UV irradiation during meiosis in yeast (Saccharomyces cerevisiae)
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 146 (1) , 55-59
- https://doi.org/10.1007/bf00267983
Abstract
Irradiation of meiotic yeast cells with moderate doses of ultraviolet irradiation (1,600 erg/mm2) leads to the arrest of premeiotic DNA synthesis, massive (5–40%) DNA degradation, and a 40–50% loss of cell viability. In contrast, such doses of UV irradiation had a minor effect on viability (15–20% loss) of logarithmically growing cells, and no comparable DNA degradation was observed in irradiated synchronized vegetative cells. Meiotic recombination is also affected by UV irradiation. When administered at a stage comparable to meiotic prophase, low doses of irradiation result in a reduction in recombination frequency without significantly affecting cell viability.Keywords
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