HISTOCHEMICAL-OBSERVATIONS ON MUSCLE FROM NORMAL AND MALIGNANT HYPERTHERMIA-SUSCEPTIBLE SWINE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (7) , 1069-1071
Abstract
A comparison was made of muscle from 2 locations in both the longissimus and the semitendinous muscles of normal and malignant hyperthermia-susceptible swine. Serial frozen sections were stained for alkali-stable ATPase, phosphorylase, and the oxidative enzymes succinate dehydrogenase and reduced NADH diaphorase. Myofiber types were identified on the basis of these staining reactions. There was no consistent statistically significant difference between muscle from normal and muscle from susceptible swine with any system of fiber classification. This is contrary to several published reports, but consistent with physiologic studies which indicate that both oxidative and glycolytic pathways are abnormally active during the onset of malignant hyperthermia.