A Noninvasive Method for the Study of Hepatic Drug Metabolism in Rodents: Aminopyrine Metabolism in Rats

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Abstract
A rapid, sensitive, and simple high-performance liquid chromatography method is described for the direct analysis of aminopyrine in saliva. The detection limit was 12 ng/ml of sample. Accuracy and precision were maintained with as little as 1 .mu.l of saliva. The rate of elimination of aminopyrine was monitored noninvasively in rats. The aminopyrine half-life [t1/2] was 59.5 .+-. 10.5 min. The t1/2 was independent of aminopyrine 50-200 mg/kg. In rats single i.p. doses of SKF 525-A [proadifen] (20 mg/kg) produced increases in aminopyrine t1/2 and salivary aminopyrine concentration. Phenobarbital induction lowered the aminopyrine t1/2 to 33.2 .+-. 8.5 min and decreased the salivary aminopyrine concentration.