Interaction of wild-type and mutant M protein of vesicular stomatitis virus with nucleocapsids in vitro

Publisher Website
Google Scholar
Abstract
The interactions between mutant or wild-type M [matrix] protein and nucleocapsids of vesicular stomatitis virus (VSV) were characterized by assaying for inhibition of in vitro transcriptase activity. The interactions are primarily electrostatic in nature: high concentrations of NaCl or poly(L-glutamic acid) reverse the inhibition. These interactions are much weaker in each of the 4 M protein mutants (complementation group III) tested than in wild-type VSV. Temperature-insensitive revertants were selected from each of the M protein mutants studied. The salt-dependent inhibitory profiles of all the revertants resemble that of wild-type VSV, suggesting that M-nucleocapsid interactions are integrally related to the temperature-sensitive phenotype of group III mutants. These results are discussed in relation to an accompanying paper which shows that interaction between M protein and infected cell membranes is increased in all group III mutants studied.