Partial Purification of Gibberellin Oxidases from Spinach Leaves
- 1 September 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 85 (1) , 87-90
- https://doi.org/10.1104/pp.85.1.87
Abstract
Four enzyme activities catalyzing the following oxidative steps in the gibberellin (GA) biosynthetic pathway have been extracted from spinach (Spinacia oleracea L.) leaves after exposure to 8 long days: GA12 .fwdarw. GA53 .fwdarw. GA44 .fwdarw. GA19 .fwdarw. GA20. Two of these, GA53 oxidase and GA19 oxidase, were separable from the other two, GA44 oxidase and GA12 13-hydroxylase, by anion exchange high performance liquid chromatography (HPLC). Apparent molecular weights of the four enzymes as determined by gel filtration HPLC are: GA12 13-hydroxylase, 28,400; GA53 oxidase, 42,500; GA44 oxidase, 38,100; GA19 oxidase, 39,500. GA44 oxidase was purified approximately 100-fold in 0.3% yield by a combination of ammonium sulfate fractionation, anion exchange HPLC, phenyl-Sepharose chromatography and gel filtration HPLC.This publication has 12 references indexed in Scilit:
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