Preparation of [125I-Tyr27,Leu5]beta h-endorphin and its use for crosslinking of opioid binding sites in human striatum and NG108-15 neuroblastoma-glioma cells.
- 1 July 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (13) , 4622-4625
- https://doi.org/10.1073/pnas.83.13.4622
Abstract
A radioligand suitable for crosslinking studies to opoid receptors has been obtained by radioiodination and purification of the monoiodotyrosine-27 derivative of the synthetic human .beta.-endorphin (.beta.h-endorphin) analogue [5-leucine].beta.h-endorphin. The derivative, [27-[125I]monoiodotyrosine, 5-leucine].beta.h-endorphin, was crosslinked to human striatal (caudate and putamen) and NG105-15 neuroblastoma-glioma cell membranes by using disuccinimidyl suberate. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions revealed four specifically labeled bands at 68, 40, 30, and 25 kDa for both human caudate and putamen, whereas NG108-15 cell membranes gave specifically labeled bands at 92, 56, 38, and 23 kDa.This publication has 34 references indexed in Scilit:
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