Nineteen new microsatellite DNA polymorphisms in pigtailed macaques (Macaca nemestrina)
- 1 July 2000
- journal article
- Published by Springer Nature in Primates
- Vol. 41 (3) , 343-350
- https://doi.org/10.1007/bf02557603
Abstract
Microsatellite loci known to be polymorphic in baboons (Papio hamadryas) and/or humans were tested in pigtailed macaques (Macaca nemestrina) from the Washington Regional Primate Research Center. Nineteen polymorphisms were identified in the macaques, with an average of 9.2 alleles per locus and an average heterozygosity of 0.76. Seven loci were analyzed using radiolabelled PCR primers and standard gel electrophoresis. Twelve loci were studied using fluorescently labelled primers and the Perkin-Elmer ABI 377 genotyping system. Of these 19 pigtailed macaque polymorphisms, 12 were used to perform paternity testing among captive animals. In a set of 15 infants, this panel of 12 genetic polymorphisms was sufficient to establish paternity in all cases. The number of alleles per locus in pigtailed macaques was compared with the number of alleles in a sample of baboons, and no significant correlation was observed. This indicates that population genetic processes such as genetic drift and recurrent mutation act rapidly enough on these loci to eliminate any relationship in levels of polymorphism across those two species. These 19 loci will be valuable for a range of genetic studies in pigtailed macaques, including paternity testing, analysis of population structure and differentiation among wild populations, and genetic linkage mapping.Keywords
This publication has 33 references indexed in Scilit:
- Rapid Screening and Comparison of Human Microsatellite Markers in Baboons: Allele Size Is Conserved, but Allele Number Is NotGenomics, 1998
- Primer NotesMolecular Ecology, 1998
- Advantages and limitations of nonhuman primates as animal models in genetic research on complex diseasesJournal of Medical Primatology, 1997
- Human Microsatellites Applicable for Analysis of Genetic Variation in Apes and Old World MonkeysJournal of Heredity, 1996
- Conservation of human chromosome 18 in baboons (Papio hamadryas):a linkage map of eight human microsatellitesCytogenetic and Genome Research, 1996
- Linkage Mapping in Papio Baboons: Conservation of a Syntenic Group of Six Markers on Human Chromosome 1Genomics, 1995
- Myotonic dystrophy: An unstable CTG repeat in a protein kinase geneSeminars in Cell Biology, 1995
- Relative size and evolution of the germline repertoire of T-cell receptor β-chain gene segments in nonhuman primatesGenomics, 1995
- Paternity exclusion in a community of wild chimpanzees using hypervariable simple sequence repeatsMolecular Ecology, 1994
- Genetic differentiation between baboon subspecies: Relevance for biomedical researchAmerican Journal of Primatology, 1990