DEVELOPMENT OF A NEW MELANOMA MODEL IN C57BL/6 MICE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (8) , 3157-3163
Abstract
Melanomas were induced in C57BL/6 mice by a single application of 7,12-dimethylbenz(a)anthracene to the scapular region of 4-day-old mice, followed by twice-weekly applications of croton oil. Of 20 mice treated, melanomas arose in 2 female littermates. The 1st melanoma (JB/MS) arose 16 wk after initiation of treatment, and the 2nd melanoma (JB/RH) arose 23 wk later. The melanomas maintained their melanotic appearance after s.c. transplantation to normal C57BL/6 mice and metastasized spontaneously in the transplant recipients. These are thought to be the 1st melanomas to have been induced in C57BL/6 mice since the B16 melanoma arose spontaneously in 1954. The JB/MS and JB/RH melanomas provide an excellent comparative system for studies done with the B16 melanoma. These melanomas of recent origin will also facilitate the investigation of biological, immunological and biochemical parameters that influence the growth and metastasis of malignant melanomas.