Evaluation of Two-Dimensional Polyacrylamide Gel Electrophoresis of Acidic Proteins of Ribosome Preparations for Identifying Plant Pathogenic Soft-Rotting Bacteria
Two-dimensional polyacrylamide gel electrophoretic separation of proteins was successfully used for identifying cultures of plant pathogenic bacteria. Profiles of soluble proteins were complex; profiles of ribosomal protein-enriched fractions contained only 5-10 major proteins and could be used to distinguish strains of Erwinia carotovora. E. atroseptica and E. chrysanthemi. Most significant differences in profiles of ribosomal-enriched protein of Erwinia spp. were in those of the acidic proteins in the 20-50 Kdalton MW range. Although several proteins were similar on all strains of E. carotovora and E. atroseptica examined, significantly different clusters were observed between these strains and those of E. chrysanthemi.