Determinants of the C-Terminal Domain of the Escherichia coli RNA Polymerase α Subunit Important for Transcription at Class I Cyclic AMP Receptor Protein-Dependent Promoters
- 15 April 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (8) , 2273-80
- https://doi.org/10.1128/jb.184.8.2273-2280.2002
Abstract
Alanine scanning of the Escherichia coli RNA polymerase α subunit C-terminal domain (αCTD) was used to identify amino acid side chains important for class I cyclic AMP receptor protein (CRP)-dependent transcription. Key residues were investigated further in vivo and in vitro. Substitutions in three regions of αCTD affected class I CRP-dependent transcription from the CC(−61.5) promoter and/or the lacP1 promoter. These regions are (i) the 287 determinant, previously shown to contact CRP during class II CRP-dependent transcription; (ii) the 265 determinant, previously shown to be important for αCTD-DNA interactions, including those required for class II CRP-dependent transcription; and (iii) the 261 determinant. We conclude that CRP contacts the same target in αCTD, the 287 determinant, at class I and class II CRP-dependent promoters. We also conclude that the relative contributions of individual residues within the 265 determinant depend on promoter sequence, and we discuss explanations for effects of substitutions in the 261 determinant.Keywords
This publication has 32 references indexed in Scilit:
- Architecture of fis-activated transcription complexes at the Escherichia coli rrnB P1 and rrnE P1 promotersJournal of Molecular Biology, 2002
- The C-terminal domains of the RNA polymerase α subunits: contact site with fis and localization during co-activation with CRP at the Escherichia coli proP P2 promoterJournal of Molecular Biology, 2002
- Interaction of the C-terminal domain of the E. coli RNA polymerase α subunit with the UP element: recognizing the backbone structure in the minor groove surface11Edited by R. EbrightJournal of Molecular Biology, 2001
- Transcription activation by catabolite activator protein (CAP)Journal of Molecular Biology, 1999
- The c-terminal domain of the alpha subunit of Escherichia coli RNA polymerase is required for efficient rho-dependent transcription terminationJournal of Molecular Biology, 1998
- Activation of P2 late transcription by P2 ogr protein requires a discrete contact site on the C terminus of the α subunit of Escherichia coli RNA polymeraseJournal of Molecular Biology, 1997
- Flexible linker in the RNA polymerase alpha subunit facilitates the independent motion of the C-terminal activator contact domainJournal of Molecular Biology, 1997
- Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP) II. Role at class I and class II CAP-dependent promotersJournal of Molecular Biology, 1994
- Mapping the cAMP receptor protein contact site on the α subunit of Escherichia coli RNA polymeraseMolecular Microbiology, 1992
- Deletion mutagenesis of the Escherichia coli galactose operon promoter regionJournal of Molecular Biology, 1983