Bridging Reagent for Protein
- 1 October 1967
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 62 (4) , 419-423
- https://doi.org/10.1093/oxfordjournals.jbchem.a128684
Abstract
1.N6, N6-Hexamethylenebiscarbamoyllysine was synthesized by treating the copper complex of lysine with hexamethylenediisocyanate. The properties of this compound were described. 2. Ribonuclease [EC 2.7.7.16] was treated with hexamethylenediisocyanate. The derivative, in which 1.8 moles of the lysine residues reacted with hexamethylenediisocyanate, had the full activity. The fully active derivative had similar intrinsic viscosity and sedimentation coefficient to that of ribonuclease. It seemed therefore to have one intra-molecular bridge of hexamethylenediisocyanate. 3.Chymotrypsin [EG 3.4.4.5] treated with hexamethylenediisocyanate was obtained as an insoluble and enzymatically active derivative. The activity of the insoluble fraction was about 30%of the original.This publication has 1 reference indexed in Scilit:
- Preparation and Properties of Water-insoluble Derivatives of TrypsinJournal of Biological Chemistry, 1963