Interaction of Ras with phosphoinositide 3-kinase γ
- 15 September 1997
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 326 (3) , 891-895
- https://doi.org/10.1042/bj3260891
Abstract
Phosphoinositide 3-kinase γ (PI3Kγ) can be activated in vitro by both α and βγ subunits of heterotrimeric G-proteins and does not interact with p85, the regulatory subunit of PI3Kα. Here we demonstrate the binding of Ras to PI3Kγ in vitro. An N-terminal region of PI3Kγ was identified as a binding site for Ras. After co-expression with PI3Kγ in COS-7 cells, Ras induced only a modest increase in PI3K activity compared with the stimulation of PI3Kα by Ras in the same cells.Keywords
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