HL-A Expression on the G 1 S, and G 2 Cell-Cycle Stages of Human Lymphoid Cells

Publisher Website
Google Scholar
Abstract
Human lymphoid culture cells were separated according to size and corresponding cell cycle phase with a velocity sedimentation method in which a continuous 5–20% (wt/wt) Ficoll gradient was used. With this method the expression of HL-A antigens on the cell-cycle stages of the human culture line RPMI 8866, which bears HL-A2, 3, and 7 specificities, was examined. The presence of antigen was determined by specific inhibition with these cells of cytotoxicity for human peripheral blood lymphocytes, with alloantisera for HL-A2, 3, and 7. Nonspecificity of the reactions was controlled with alloantisera for HL-Al, 8, and W5. The total surface-absorbing capacity was increased in Sand G2 compared to that in G1. The absorbing capacity per cell-surface area (antigen density) appeared also to be increased in G2. This relative increase in total antigen and antigen density in Sand G2 phases was corroborated by similar findings with the use of log-phase (S-enriched) lymphoid cultures as compared with stationary-phase (G0- or G1-enriched) cultures in the direct cytotoxicity and inhibition of cytotoxicity assays.

This publication has 0 references indexed in Scilit: