PLATELETS INCREASE NEUTROPHIL ADHERENCE INVITRO TO NYLON FIBER

  • 1 January 1981
    • journal article
    • research article
    • Vol. 97  (6) , 812-819
Abstract
The interaction of neutrophils with platelets may be important in the pathogenesis of various diseases including thrombus and vascular lesions. The effect of platelets on the adherence of neutrophils to nylon fiber was assessed in human whole blood samples and purified neutrophil suspensions in the presence or absence of plasma. In whole blood samples, increasing numbers of platelets were associated (r = 0.47, P < 0.02) with increasing adherence of neutrophils. Addition of platelets in plasma to purified neutrophil suspensions increased (P < 0.05) neutrophil adherence from 76.2% .+-. 1.4 to 88.0% .+-. 2.0. Addition of washed platelets without plasma also increased (P < 0.05) neutrophil adherence from 67.9% .+-. 5.8 (without added platelets) to 94.2% .+-. 1.6 (with 300,000 platelets/mm3 added). No augmentation of neutrophil adherence occurred if platelets had their aggregation response suppressed by pretreating platelet donors with aspirin. Scanning electron microscopy supported these findings by showing platelets in close association with neutrophils adhering to nylon fiber. The importance of platelet numbers and reactivity on the adherence of neutrophils is emphasized.

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