Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1
- 22 July 1993
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 364 (6435) , 308-313
- https://doi.org/10.1038/364308a0
Abstract
In higher eukaryotes, the Ras and Raf-1 proto-oncoproteins transduce growth and differentiation signals initiated by tyrosine kinases. The Ras polypeptide and the amino-terminal regulatory domain of Raf-1(residues 1–257) are shown to interact, directly in vitro and in a yeast expression system. Raf-1(1-257) binds GTP-Ras in preference to GDP-Ras, and inhibits Ras-GAP activity. Mutations in and around the Ras effector domain impair Ras binding to Raf-1(1-257) and Ras transforming activity in parallel.Keywords
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