The Localization of the Cell-Bound Penicillinase of Bacillus cereus in Protoplasts

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Abstract
Protoplasts of Bacillus cereus 569 and B. cereus 569/H were shown to be as competent as intact cells in the induced and constitutive formation of penicillinase, as well as in other biochemical activities. It was demonstrated that the cell-bound penicillinase was localized at or near the cell surface. The [beta]-penicillinase fraction was released by digestion of the cell wall and by washing of the protoplast membrane. However, the gamma-penicillinase was tightly bound to the cytoplasmic membrane. It was obtained in a soluble form by treating the membrane with sonic vibration or with fat solvents.