Substrate-assisted catalysis of peptide bond formation by the ribosome
- 10 October 2004
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 11 (11) , 1101-1106
- https://doi.org/10.1038/nsmb841
Abstract
The ribosome accelerates the rate of peptide bond formation by at least 107-fold, but the catalytic mechanism remains controversial. Here we report evidence that a functional group on one of the tRNA substrates plays an essential catalytic role in the reaction. Substitution of the P-site tRNA A76 2′ OH with 2′ H or 2′ F results in at least a 106-fold reduction in the rate of peptide bond formation, but does not affect binding of the modified substrates. Such substrate-assisted catalysis is relatively uncommon among modern protein enzymes, but it is a property predicted to be essential for the evolution of enzymatic function. These results suggest that substrate assistance has been retained as a catalytic strategy during the evolution of the prebiotic peptidyl transferase center into the modern ribosome.Keywords
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