Extensive conjugation of dopamine (3,4-dihydroxyphenethylamine) metabolites in cultured human skin fibroblasts and rat hepatoma cells
- 15 October 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 176 (1) , 187-196
- https://doi.org/10.1042/bj1760187
Abstract
[G-3H]Dopamine metabolism in human skin fibroblasts and rat hepatoma cells in culture was determined by high-pressure liquid-chromatographic analysis of both cell extract and uptake medium. Conjugated metabolites were selectively hydrolyzed by incubation with arylsulfatase [EC 3.1.6.1] or .beta.-glucuronidase before analysis. The principal metabolites of dopamine in fibroblast cells are 3-methoxytyramine 4-O-sulfate and 3-methoxytyramine. No significant differences, either in the amounts of these metabolites or in the amount of dopamine metabolism, were observed in fibroblasts from both normal and homocystinuric individuals. In rat hepatoma cells, the major metabolite of dopamine was 3-methoxytyramine 4- or 3-O-glucuronide; lower concentrations of dopamine 4- or 3-O-glucuronide, 4-hydroxy-3-methoxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid and 2 unidentified glucuronide conjugates were also observed. Significant differences in the relative concentrations of these metabolites in cell and uptake medium were observed in both cell systems.This publication has 32 references indexed in Scilit:
- MONOAMINE OXIDASE A AND B IN CULTURED CELLSJournal of Neurochemistry, 1978
- Conjugated Catechol Derivatives in a Transplantable Islet Cell Tumour of the Golden HamsterActa Physiologica Scandinavica, 1977
- Monoamine oxidase and catechol-O-methyltransferase activities in cultured human skin fibroblastsLife Sciences, 1976
- Monoamine Oxidase Activity Decreased in Cells Lacking Hypoxanthine Phosphoribosyltransferase ActivityScience, 1976
- A method for the preparation and separation of 3-methoxy-4-hydroxyphenethyleneglycol conjugatesBiochemical Medicine, 1975
- RESERPINE SENSITIVITY OF CATECHOLAMINE METABOLISM IN MURINE NEUROBLASTOMA CLONE N1E‐1151Journal of Neurochemistry, 1975
- Measurement of the two conjugates of 3-methoxy-4-hydroxyphenylglycol in urineBiochemical Medicine, 1974
- Sulphate Conjugation and L-Dopa Treatment of Parkinsonian PatientsNature, 1973
- Homocystinuria associated with decreased methylenetetrahydrofolate reductase activityBiochemical and Biophysical Research Communications, 1972
- Dopamine (3-Hydroxytyramine) metabolism in parkinsonismJournal of Clinical Investigation, 1969