Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.
- 1 September 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (17) , 5875-5879
- https://doi.org/10.1073/pnas.82.17.5875
Abstract
The 2-.mu.m circle DNA of yeast encodes a site-specific recombination system (FLP recombination). The recombination region had been mapped earlier to a 65-base-pair (bp) segment within the 599-bp-long inverted repeats of the molecule. I have shown that the "minimal" FLP substrate resides in a 13-bp dyad symmetry plus an 8-bp core located within the 65-bp recombination region. Further, as determined by different in vivo assays, sequences extraneous to the minimal FLP site and the 65-bp recombination region can affect the efficiency of the recombination reaction.Keywords
This publication has 29 references indexed in Scilit:
- Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancerCell, 1985
- The FLP recombinase of the 2μ circle DNA of yeast: Interaction with its target sequencesCell, 1985
- Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination systemJournal of Molecular Biology, 1985
- Recombination site selection by Tn3 resolvase: Topological tests of a tracking mechanismCell, 1985
- The mechanism of phage λ site-specific recombination: Site-specific breakage of DNA by Int topoisomeraseCell, 1983
- Recombination within the yeast plasmid 2μ circle is site-specificCell, 1982
- Transposon-mediated site-specific recombination: A defined in vitro systemCell, 1981
- Phase Variation: Evolution of a Controlling ElementScience, 1980
- Replication and recombination functions associated with the yeast plasmid, 2μ circleCell, 1980
- Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene, 1979