A New Method for Elution of Erythrocyte-Bound Antibody

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Abstract
A new method is described for eluting antibody from erythrocytes. Sensitised erythrocytes are subjected to ultrasonic waves; elution probably depends on the application both of heat and great mechanical force. It was successfully used to elute human and animal IgG and IgM antibodies and seed agglutinins. By direct application of ultrasound to sensitised erythrocytes the time taken to produce strong eluates ranged from 30 sec for some seed agglutinins to 10 min for IgG anti-D.

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