DEVELOPMENT AND CHARACTERIZATION OF A HUMAN SARCOMA CELL-LINE, MES-SA, SENSITIVE TO MULTIPLE-DRUGS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (10) , 4943-4950
Abstract
A cell line designated MES-SA was developed from a uterine sarcoma. Cells from the surgical tumor specimen were grown in a soft-agar clonogenic assay, with a relatively high plating efficiency of 0.5% and sensitivity to multiple drugs. Histologically, the surgical specimen and tumors developing after MES-SA inoculation into nude mice were identical, consisting of sheets of anaplastic sarcoma cells amid scant hyalinized stroma. The nonepithelial origin of this line was supported by ultrastructural analysis and negative mucin staining. Growth in monolayer was established by seeding colonies from soft agar into liquid media and has been maintained for > 21 mo. (> 100 passages), with a population-doubling time for the cell line of 22 h. The MES-SA line readily forms colonies in soft agar with plating efficiencies from 10-20%. Tumor cell inoculation s.c. into nude mice produces tumors within 2-3 wk and subsequent tumor volume-doubling times of 7-10 days. MES-SA has a modal chromosome number of 45. Karyotypic abnormalities include the following: monosomic forms of chromosomes 5, 6 and 7; a 5q, 6p translocation; and 1 marker chromosome. In vitro sensitivities to doxorubicin, dactinomycin, mitomycin C and bleomycin were demonstrated by clonogenic assay. These drugs sensitivities remain stable over long periods of monolayer growth and after passage in nude mice.

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