The influence of polycyclic aromatic hydrocarbons on bacteriophage development. II.

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Abstract
A method is described for preparing infected spheroplasts from cells preinfected with phage for use in testing the hydrocarbon effect on viral replication. In cells infected with single-stranded viruses, a close correlation is observed between aromatics that inhibit virus replication and those that are carcinogenic. This relationship, however, is not apparent when double-stranded viruses are used in the same test system. The powerful carcinogen, 7,12-dimethyl-benz(a)anthracene inhibits nucleic acid and protein synthesis specific for viral replication as well as the same synthesis in noninfected cells. Viral replication can be partially protected from 7,12-DMBA by the presence of other aromatics which are not themselves viral inhibitors or carcinogenic inducers.