Rapid and Sensitive Determination of Thalidomide in Human Plasma by High Performance Liquid Chromatography

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Abstract
A sensitive and rapid High-performance liquid chromatographic method using U. V. detection, has been developed for the analysis of thalidomide in plasma. This involved a single liquid-solid extraction on Extra-Sep-C8 column in the presence of an internal standard (ciprofloxacin). Analysis was performed by isocratic elution with a mobile phase consisted of 0.01 M aqueous potassium dihydrogen phosphate containing 21% (V/V) acetonitrile and 4.5 mM Heptane sulfonic acid, adjusted to pH 2.3, with U. V detection at 295 nm. The limit of sensitivity of the assay was 0.06 mg/l. The method was applied to a pharmacokinetic study (50 to 100 mg) in patients with erythema nodusum leprosum (ENL) with a good accuracy (96–111%) and precision (less than 5.8%).