The Occurrence of Acetylcholine in the Nervous Tissue of American Cockroaches1

Abstract
The presence of acetylcholine in the nerve tissue of American cockroaches was investigated. The nerve tissue was repeatedly extracted with ethanol and acetylcholine was separated by paper chromatography. Acetylcholine was demonstrated in the roach nerve tissue both chemically and pharmacologically. Acetylcholine is present in potentially effective concentration, 41.5 µg per gram of nerve tissue and this accounts for all the pharmacological activity of the original nerve extract. In addition to acetylcholine, the roach nerve tissue extracted by these methods contains choline, tyrosine and histidine.

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