Antifertility Effects of Immunoglobulins from Uterine Fluids of Semen-Immunized Rabbits
Open Access
- 1 May 1974
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 10 (4) , 422-428
- https://doi.org/10.1095/biolreprod10.4.422
Abstract
Fluids were collected from the ligated and stimulated uterine horns of rabbits that had been isoimmunized with semen and adjuvant both transvaginally and systemically or only transvaginally. Treating rabbit sperm with immune uterine fluids (IUF) decreased fertility in inseminated rabbits. The antifertility effect of IUF was attributed to antibody activity as it was removed by absorption with sperm and goat antisera against rabbit gamma globulins. The predominant immunoglobulins, IgC and secretory IgA, were separated from IUF by gel filtration Sephadex G-200 and by anion exchange on DEAE cellulose. The separated immunoglobulins were also treated with monospecific antisera against both Ig classes to remove possible cross contamination. The results indicated that both IgG and secretory IgA in IUF possessed specific sperm antibodies capable of depressing fertility in inseminated rabbits.Keywords
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