Biologic activity in a fragment of recombinant human interferon alpha.
- 1 February 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (4) , 1045-1047
- https://doi.org/10.1073/pnas.81.4.1045
Abstract
To locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-.alpha.2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced 2 major complementary fragments, HuIFN-.alpha.2-(1-110) and HuIFN-.alpha.2-(111-153). After reduction with 2-mercaptoethanol and separation of the 2 major fragments on NaDodSO4/polyacrylamide gel electrophoresis, antiviral activity persisted in the larger, MW 12,000, fragment consisting of the amino-terminal 110 amino acids.This publication has 23 references indexed in Scilit:
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