Titration of Influenza Virus and Viral Antibodies by Preserved Human Erythrocytes.

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Abstract
Previous work in this laboratory demonstrated effective preservation of sheep erythrocytes for use in various serological systems. The preparation of preserved human erythrocytes and their use in both tube and rapid plate hemagglutination and hemagglutination-inhibition procedures for the titration of influenza virus and viral antibodies are described. Such preserved cells were stable, with respect to both resistance to lysis and sensitivity to hemagglutination by influenza virus, for a year. Preserved human erythrocytes showed good correlation in sensitivity with freshly washed human erythrocytes, and the rapid plate technique using preserved human erythrocytes correlated well with tube hemagglutination and hemagglu-tination-inhibition procedures.