Complex Formation by the mrpABCDEFG Gene Products, Which Constitute a Principal Na + /H + Antiporter in Bacillus subtilis
Open Access
- 15 October 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (20) , 7511-7514
- https://doi.org/10.1128/jb.00968-07
Abstract
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na + /H + antiporter encoded by mrpABCDEFG . Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.Keywords
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