Reversibility of Protein Binding of Penicillins: an In Vitro Study Employing a Rapid Diafiltration Process

Abstract
The reversibility of human serum protein binding of oxacillin, nafcillin, and cephalothin was investigated by using a diafiltration technique. While the temperature, p H, and protein concentration were kept constant, 20% of the serum volume was exchanged every 4 to 5 min. The binding of all three drugs was rapidly reversible, and the percentage of protein binding remained the same throughout the experiment.