Approaches to Solid Phase DNA Sequencing
- 1 October 1988
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 7 (5-6) , 629-638
- https://doi.org/10.1080/07328318808056299
Abstract
A novel approach to enable two-directional solid-phase DNA sequencing, involving immobilization of double stranded plasmid to avidin agarose, is described. A plasmid vector, pRIT28, has been designed to allow enzymatic incorporation of biotinylated dUTP into two alternative sites.Keywords
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