Zur Bestimmung von Fettsäureestern in Blut- und Organfetten

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Abstract
An adaptation of the method employing conversion of the fatty acid esters to hydroxamic acids and subsequent colorimetric estimation of these by means of their ferric complexes is worked out. To the extracted esters, in 4 ml of a 1:1 mixture of methanol: diethyl ether, is added 1 ml of a glycine-sodium hydroxide-hydroxylamine solution. The reaction is allowed to proceed at room temperature in the dark for 7-24 hours. After addition of perchloric acid and ferric chloride the colors produced are then read at 530-540 mu. Aldehydes, peroxides and similar substances are the chief sources of error and are carefully removed from the organic solvents employed. The method allows the estimation 1-50 micro-equivalents; the error is 3%.

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