Histochemical Localization of L-Gulonolactone Oxidase Activity in Tissues of Several Species.
- 1 February 1961
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 106 (2) , 309-311
- https://doi.org/10.3181/00379727-106-26319
Abstract
This paper describes a method for the histochemical demonstration of L-gulonolactone oxidase activity. Its principle depends on the reduction of a tetrazolium salt at the site of activity. The centrilobular cells of the rat liver, and selected portions of the frog kidney showed activity. Guinea pig liver and frog liver were negative. The oxidation of L-gulonolactone is the final step in the synthesis of ascorbic acid from glucose.Keywords
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