Parabiotic Cell Culture. IV. Interaction Between Normal and Ascites Tumor Cells of Rats2

Publisher Website
Google Scholar
Abstract
With the use of the parabiotic cell culture method, interaction between the cells from normal tissue (liver cells, heart fibroblasts, and kidney epithelial cells) and transplantable ascites tumor cells (Hepatoma AH-130 and Yoshida sarcoma cells) of rats was examined quantitatively by cell counting. The experiments were planned to reproduce conditions in vivo of tumor growth, e.g., a situation in which tumor cells are rapidly proliferating and a primary culture of normal cells is maintained at a constant population level. In the combination of liver and hepatoma cells, or fibroblasts and sarcoma cells, the proliferation of tumor cells was apparently accelerated in the parabiotic culture, while normal cells were inhibited or destroyed. The fact that this interaction was further accelerated when these cells were cultivated together in a single tube suggests the possibility of direct action by cell contact. In other combinations between these normal and tumor cells, little interaction was observed.