Immune Response to Haptenated Syngeneic and Allogeneic Lymphocytes.

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Abstract
The B [bone marrow derived] cell immune response to altered self was studied using fluoresceinated syngeneic and allogeneic lymphocytes as antigens. Antibody synthesis could be induced in mice injected with syngeneic or allogeneic haptenated lymphocytes and heterologous labeled red cells using 1 particular hapten density. The response to haptenated syngeneic lymphocytes was thymus-independent since nude mice gave a strong response even when the immunogen was FITC[fluorescein isothiocyanate]-labeled nude cells. The antibodies induced reacted with a complex between a self structure and the hapten, since the best response was detected using FITC-labeled mouse erythrocytes as targets in the plaque assay. A clear distinction must be made between recognition and activation of the responding B cells. The nature of the structures on the modified lymphocyte required to perform these 2 functions is discussed. In a dual recognition model of altered self, the duality involved may concern recognition and activation events, rather than 2 recognition receptors.