500 MHz Proton NMR evidence for two solution structures of the common arm base-paired segment of wheat germ 5S ribosomal RNA
- 20 February 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 29 (7) , 1722-1730
- https://doi.org/10.1021/bi00459a009
Abstract
The base-pair protons of the common arm duplex fragment of wheat germ (Triticum aestivum) ribosomal 5S RNA have been identified and assigned by means of 500-MHz proton NMR spectroscopy. The two previously reported extra base pairs within the fragment [Li et al. (1987) Biochemistry 26, 1578-1585] are now explained by the presence of two distinct solution structures of the common arm fragment (and its corresponding base-paired segment in intact 5S rRNA). The present conclusions are supported by one- and two-dimensional proton homonuclear Overhauser enhancements in H2O and by temperature variation and Mg2+ titration of the downfield 1H NMR spectrum. The difference between the two conformers is most likely due to difference in helical tightness. Some additional amino proton resonances have also been assigned.This publication has 2 references indexed in Scilit:
- Structure and Dynamics of BiopolymersPublished by Springer Nature ,1987
- NMR studies of ion binding to Escherichia coli tRNAPheBiochemistry, 1985