Effects of DL‐α‐difluoromethylornithine on the growth and metastasis of b16 melanoma in vivo

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Abstract
The effects of DL‐α‐difluoromethylornithine (DFMO), a specific irreversible inhibitor of ornithine decarboxylase, on the growth of experimental mouse B16‐F10 melanoma cells were investigated. DFMO (3%) in drinking water was administered to B16‐F10 melanoma‐bearing mice. At 24 days, B16‐F10 melanomas in DFMO‐fed mice weighed 75% less than those in control mice (p < 0.001). DFMO reduced putrescine and spermidine levels in B16‐F10 melanoma by 98% and 84%, respectively, and prolonged the mean survival time from 25.9 ± 1.2 to 35.7 ± 2.2 days (p < 0.001). The effects of DFMO on experimental metastasis were also investigated. DFMO treatment resulted in a significant decrease in pulmonary metastasis induced by i.v. injection of B16‐F10 melanoma cells.