Relative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations
Open Access
- 1 July 2008
- journal article
- research article
- Published by Springer Nature in Journal of Translational Medicine
- Vol. 6 (1) , 34
- https://doi.org/10.1186/1479-5876-6-34
Abstract
Quantification of T-cell receptor (TCR) chain families can be utilized for detection of clonal T-cell populations. Besides southern blotting and antibody-based approaches, quantitative real time PCR (qRT PCR) has been more widely applied in this context during the last years. Here, the heterogeneity of sequences within single families is the most challenging problem for exact quantification.Keywords
This publication has 19 references indexed in Scilit:
- Analysis of the CDR3 length repertoire and the diversity of TCR alpha chain in human peripheral blood T lymphocytes.2007
- Standardized analysis for the quantification of Vβ CDR3 T-cell receptor diversityJournal of Immunological Methods, 2006
- HUMAN T CELL RESPONSES AGAINST MELANOMAAnnual Review of Immunology, 2006
- T cell receptor BV gene repertoire of lymphocytes in bronchoalveolar lavage fluid of polymyositis/dermatomyositis patients with interstitial pneumonitisInternational Journal of Molecular Medicine, 2006
- Cytotoxic T CellsJournal of Investigative Dermatology, 2006
- New methods and software tools for high throughput CDR3 spectratyping. Application to T lymphocyte repertoire modifications during experimental malariaJournal of Immunological Methods, 2003
- Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency AnalysisClinical and Vaccine Immunology, 2002
- Detection and Characterization of αβ-T-Cell Clonality by Denaturing Gradient Gel Electrophoresis (DGGE)BioTechniques, 1998
- Human T-cell receptor variable gene segment familiesImmunogenetics, 1995
- T-cell antigen receptor genes and T-cell recognitionNature, 1988