Nitrite-induced volatile mutagens from normal human feces
- 1 March 1981
- Vol. 47 (5) , 889-894
- https://doi.org/10.1002/1097-0142(19810301)47:5<889::aid-cncr2820470513>3.0.co;2-r
Abstract
Volatile mutagens (putative carcinogens) were produced from normal human and animal feces upon incubation with sodium nitrite in saline at 37.degree. C for 48 h. The mutagens were detected by using Ames'' Salmonella typhimurium tester strain TA1535 without microsomes, on plates inverted over samples in sealed containers. Mutagenicity was maximal at 0.2-0.6 M NaNO2 and at pH 6.2-6.8. Reversions per plate varied from .apprx. 30-450 (1.5-25 .times. background) within the normal human population. Sodium ascorbate and .alpha.-tocopherol (at 1/2 [NaNO2]) each reduced the mutagenicity by .apprx. 30%. Two standard N-nitroso-compounds were mutagenic in the system. The mutagenicity in this system is probably caused by the formation of volatile N-nitroso-compounds. Addition of nitrite to human feces in vitro enhances a process that occurs in vivo.This publication has 21 references indexed in Scilit:
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