Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity.
Open Access
- 1 November 1990
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 9 (11) , 3631-3639
- https://doi.org/10.1002/j.1460-2075.1990.tb07574.x
Abstract
The cDNA for TTF‐1, a thyroid nuclear factor that binds to the promoter of thyroid specific genes, has been cloned. The protein encoded by the cDNA shows binding properties indistinguishable from those of TTF‐1 present in nuclear extracts of differentiated rat thyroid cells. The DNA binding domain of TTF‐1 is a novel mammalian homeodomain that shows considerable sequence homology to the Drosophila NK‐2 homeodomain. TTF‐1 mRNA and corresponding binding activity are detected in thyroid and lung. The chromosomal localization of the TTF‐1 gene has been determined in humans and mice and corresponds to chromosomes 14 and 12, respectively, demonstrating that the TTF‐1 gene is not located within previously described clusters of homeobox‐containing genes.This publication has 39 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- Reactivation of thyroglobulin gene expression in transformed thyroid cells by 5-azacytidineCell, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- The structure and function of the homeodomainBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1989
- DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9Cell, 1989
- A tissue-specific transcription factor containing a homeodomain specifies a pituitary phenotypeCell, 1988
- The pituitary-specific transcription factor GHF-1 is a homeobox-containing proteinCell, 1988
- T7 lysozyme inhibits transcription by T7 RNA polymeraseCell, 1987
- Synthetic oligonucleotide probes deduced from amino acid sequence dataJournal of Molecular Biology, 1985
- Inherited Primary Hypothyroidism in MiceScience, 1981